Dr. Ahmed,

To be on the safe side, I would freeze your cut slides at -70 degrees C or
colder.  There seems to be no consensus as to whether you freeze them with
dessicant or not.

Linda Sebree, HT
University of Wisconsin Hospital & Clinics
Department of Laboratory Medicine
IHC/ISH Laboratory
A4/204-2472
600 Highland Ave.
Madison, WI  53792-2472

Phone:  (608)265-6596
FAX:  (608)263-1568

> -----Original Message-----
> From:	Dr. Naseem aHmed [SMTP:nasimas@khi.compol.com]
> Sent:	Tuesday, July 06, 1999 3:49 PM
> To:	HistoNet@pathology.swmed.edu
> Subject:	Immunohistochemistry
> 
> Hello,
>  I am doing a retrospective study on formalin fixed paraffin embedded
> block and want to do some immunostains. but i dont have the stain kit
> right now . so i want to cut the sections and place it on polylysine
> coated slides but i dont know whether there are some precaution to keep
> unstained slides for immunohistochemistry.
> Thanx
> looking forward for advice
> Dr. Naseem Ahmed
> Karachi Pakistan
> 
>