RE: Humidity chambers: part II

<< Previous Message | Next Message >>
From:"margaret blount" <>
To:"Connolly, Brett" <>, histonet <>
Date:Mon, 16 Aug 1999 09:06:29 +0100

One approach to your antibodies migrating is to get a spirit level such as is
used for leveling the tables used for pouring plates (petri dishes) set it on
top of  a slide and then level your humid chamber. You can use pipette tips to
level the chamber, just push them under the dish until the spirit level is OK.
By the way do you use a PAP pen to limit the antibody, these are invaluable and
are available from most suppliers.

Best wishes,

-----Original Message-----
From:	Connolly, Brett []
Sent:	Friday, August 13, 1999 10:46 PM
To:	'histonet'
Subject:	Humidity chambers: part II

Thanks for all your responses. The replies fall into two catagories.

1. Commercial : 
Buy the 20 slide chamber from Shandon. Or Scientific Device Lab has some and
will customize (haven't called yet). Or Histology Control Systems (new to

2. Handyman/Handywoman:
Make your own set-up from Tupperware (let's have a party) or other
plasticware, cake pans, bed pans, small coffins and whatever. Use applicator
sticks, pipets, straws, or unclaimed tibia's to support the slides. 
We are currently using the Tupperware chambers. I had bought a 2' x 4' drop
ceiling light panel (the grid style that covers the fluorescent light) and
cut it up to fit in the tupperware. This works rather well in that one can
use any sized slide and the grid provides uniform support while keeping the
slides elevated about 0.5 inches off the bottom. 

But occasionally with overnight incubations the antibodies migrate to one
end of the slides and the over end dries out so I was looking for something
else to try that was a little more stable.

Brett M. Connolly, Ph.D.
Senior Research Biologist
Dept. of Human Genetics
Merck Research Laboratories
PO Box 4
West Point, PA 19486
tel. 215-652-2501
FAX 215-652-2075

<< Previous Message | Next Message >>