Use your microscope to follow the course of stain uptake and removal on a
series of control slides.  First-hand direct observation in real time is the
best way to quickly resolve such questions.

Gary W. Gill

-----Original Message-----
From: Edna_J_Gonzalez/Powderject@powderject.com
[mailto:Edna_J_Gonzalez/Powderject@powderject.com]
Sent: August 19, 1999 12:58 PM
To: HistoNet Server
Subject: H & E Staining Method




I am working with pig skin and doing H&E regressive staining using Harris
Hematoxylin (non-acidified). The problem is that the hematoxylin is too
dark and I can't differentiate what I need, especially it is too dark in
the basal layer and the epidermis of the skin. I need a H&E Regressive
Method that will be lighter. Currently I am staining with hematoxylin for
10 minutes, which I think is too much, but this is what one of the methods
say. I know there are different H&E regressive methods (with different
times for Hematoxylin). Any suggestions?

Edna Gonzalez
PowderJect Vaccines