RE: Eosin.

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From:Ian Montgomery <>
Date:Mon, 30 Aug 1999 13:26:31 +0000
Content-Type:text/plain; charset="us-ascii"

>From: "Fisher, Jean" <>
>To: "'Ian Montgomery'" <>,
>Subject: RE: Eosin.
>Date: Fri, 27 Aug 1999 11:00:15 -0500
>MIME-Version: 1.0
>	We use an alcoholic solution of Eosin & Phloxine. We do not add
>Thymol to it & have never demonstrated bugs from it.  When we used an
>aqueous Eosin, we did add Thymol.  I've never tested an aqueous solution to
>see if bugs would grow and how soon if no preservative was added.
>	Like you, I learned this practice when practically a child.
>Jeannie Fisher

	Your reply has thrown up another question. Eosin/Phloxine. When
using this stain do you add a 'pinch' of calcium chloride just before using
the stain. Another practice I learned as a trainee.

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
G12 8QQ,
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.

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