I have been using tihs product for about 6 months and feel the same way!

Cynthia Favara
Rocky Mountain Laboratories
903 S 4th Street
Hamilton, MT 59840
ph: 406-363-9317
FAX: 406-363-9286
e-mail: cfavara@nih.gov


> ----------
> From: 	Patsy.Ruegg@UCHSC.edu[SMTP:Patsy.Ruegg@UCHSC.edu]
> Sent: 	Tuesday, August 17, 1999 11:52 AM
> To: 	denisew2@juno.com
> Cc: 	histonet@pathology.swmed.edu
> Subject: 	DAB
> 
> 
> I just wanted to plug this great DAB I have been using for the last few
> months.  It is liquid and kept in the freezer, but it does not freeze
> solid
> so you can pour it out for use each time.  Must have glycerine or
> something
> in it.  Anyway, it is good stuff, never percipitates, doesn't need
> filtering
> and is very clean and strong.  Another thing, it is pretty darn cheap.  It
> is made by Dave Brigatti's company Research Genetics 1-800-533-4363.  All
> of
> their other products I have used have been excellent as well.  I use their
> ready made pepsin digestion.  A DAKO rep recommended the DAB when I was
> having trouble with the DAKO DAB, must be good if one company recommends
> it
> over their own product, huh?  I do not work for Research Genetics or have
> any affiliation, just thought you all would like to know about some good
> stuff out there.
> Patsy Ruegg
> -----Original Message-----
> From: denise M m Long-Woodward [mailto:denisew2@juno.com]
> Sent: Thursday, August 12, 1999 11:00 AM
> To: JKotzuk@salud.unm.edu
> Cc: uvsgc@msu.oscs.montana.edu; histonet@pathology.swmed.edu
> Subject: Re: filtering DAB
> 
> 
> Dako Liquid DAB+ works great and the mixed solution can be saved for as
> long as two weeks(I don't save but some of my co-workers do)
> D Woodward, Boston, MA
> 
> 
> On Thu, 12 Aug 1999 11:55:53 -0600 "Joyce Kotzuk" <JKotzuk@salud.unm.edu>
> writes:
> > I use syringe filters, I think they're regular glass fiber 
> > non-sterile prefilters, and a 3 or 5 ml syringe. I don't know 
> > exactly what we're filtering out, but the DAB turns clear after 
> > filtering, whereas before it is slightly brown. Speaking of DAB, 
> > I've been using Vector's DAB kit, and while it is easy and safe to 
> > use, it has been browner than usual lately, and I'm beginning to 
> > worry that it may be causing excessive background, so I'm thinking 
> > of switching. Any recommendations for liquid DAB reagents? (I don't 
> > want to be weighing out powder). Thanks, Joyce Kotzuk, UNM Pathology
> > 
> > >>> Gayle Callis <uvsgc@msu.oscs.montana.edu> 08/11/99 10:01AM >>>
> > Just curious, what are people using to filter DAB before applying to 
> > 
> > tissue sections?  Microfilters, non sterile variety, for syringes? 
> > or just a filter paper, # of filter paper please, ie Whatman # or 
> > an equivalent. 
> > 
> > this is for a very small amount of DAB, 2.5 mls frequently.  
> > 
> > Gayle Callis
> > 
> > 
> > 
> 
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