Here I go again,
                  I,ve been asked to produce an LR white embedded block of
embryo chick head which  can be used for Magnetic resonance then cut on an
ordinary microtome with a disposable blade or steel blade, serial sections
of 4-5 microns.So  if anybody out there can give advice me any advice about
the
best way to go about this I would be  well pleased. I have the basic method
for processing but I need the LR White to be a bit softer than for EM, I
tried it in the oven without accelerator  using a metal mould  overnight
but  to no avail.Can you get extra large gelatin capsules large enough to
grasp in a microtome chuck head ?  Is this the best way to do this?  Thanks
in anticipation.....Jill       P>S> The Pecam antibody is working on
frozens.



Jill McVee
Histologist
St. Andrews Uni.
Scotland.