Immunobed (GMA) and xylene
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From: | Katie B <bresee98@yahoo.com> |
To: | Histonet Server <histonet@pathology.swmed.edu> |
Reply-To: | |
Date: | Wed, 18 Aug 1999 12:56:22 -0700 (PDT) |
Content-Type: | text/plain; charset=us-ascii |
Hi all!
Was wondering if anyone had any insight as to what exactly happens to
GMA (Immunobed to be specific) when exposed to xylene for an extended
time. I had some unstained sections that had been coverslipped for
some specialized image analysis. When I needed to remove the
coverslips (attached with Cytoseal I believe), I soaked them in xylene.
It took more than three days to get the coverslips off. After doing
my immuno staining (which turned out fine) I noticed that the remaining
plastic on the slide was really "flakie" as compared with those
sections I stained that had never had coverslips before. Hmmm.
Enquiring minds want to know.....
===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University
e-mail: bresee98@yahoo.com
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