Immunobed (GMA) and xylene

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From:Katie B <>
To:Histonet Server <>
Date:Wed, 18 Aug 1999 12:56:22 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

Hi all!

Was wondering if anyone had any insight as to what exactly happens to
GMA (Immunobed to be specific) when exposed to xylene for an extended
time.  I had some unstained sections that had been coverslipped for
some specialized image analysis.  When I needed to remove the
coverslips (attached with Cytoseal I believe), I soaked them in xylene.
 It took more than three days to get the coverslips off.  After doing
my immuno staining (which turned out fine) I noticed that the remaining
plastic on the slide was really "flakie" as compared with those
sections I stained that had never had coverslips before.  Hmmm.

Enquiring minds want to know.....

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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