Hi histonetters,

Does anyone out there have a good procedure for doing an H&E on a 50um
frozen section of a brain?  Right now, after fixation, we're starting in
water doing our normal routine.  Everything is quite muddy, however, but
because of the thickness of the section, I'm not sure it can be any clearer.
I'd just like to hear what other people are using for their staining times.

Thanks for any help!

Emily Yandl
eyandl@genzyme.com