H & E Staining Method

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To:"HistoNet Server" <histonet@pathology.swmed.edu>
Date:Thu, 19 Aug 1999 12:57:32 -0500
Content-Type:text/plain; charset=us-ascii

I am working with pig skin and doing H&E regressive staining using Harris
Hematoxylin (non-acidified). The problem is that the hematoxylin is too
dark and I can't differentiate what I need, especially it is too dark in
the basal layer and the epidermis of the skin. I need a H&E Regressive
Method that will be lighter. Currently I am staining with hematoxylin for
10 minutes, which I think is too much, but this is what one of the methods
say. I know there are different H&E regressive methods (with different
times for Hematoxylin). Any suggestions?

Edna Gonzalez
PowderJect Vaccines

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