Frozen secioning fat tissue
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From: | HistoScientific <histosci@shentel.net> |
To: | histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Tue, 17 Aug 1999 09:51:13 -0400 |
Content-Type: | text/plain; charset=us-ascii |
Histonetters,
We just completed a study consisting of mouse fat. This fat was taken
from around such tissues as the uterus, heart, etc. We used a cryostat
made by Microm and had it set at everywhere from -18 to -35 degrees. We
left the tissue in the cryostat overnight and still no luck. We could
not get a section no matter how thick or thin we tried cutting them. We
then tried Histofreeze, still no luck. To make a long story short, we
ended up giving "fat smears". We have never had problems with our
cryostat and the techs that were working on this project had over a
century of experience with frozens. Maybe Instrumedics could have come
to the rescue!
Beth Poole
Histo-Scientific Research Labs.
107 Killmon Road
P.O. Box 30
Basye, VA 22810
(540)856-2222
histosci@shentel.net
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