Frozen secioning fat tissue

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From:HistoScientific <>
Date:Tue, 17 Aug 1999 09:51:13 -0400
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We just completed a study consisting of mouse fat.  This fat was taken
from around such tissues as the uterus, heart, etc.  We used a cryostat
made by Microm and had it set at everywhere from -18 to -35 degrees.  We
left the tissue in the cryostat overnight and still no luck.  We could
not get a section no matter how thick or thin we tried cutting them.  We
then tried Histofreeze, still no luck.  To make a long story short, we
ended up giving "fat smears".  We have never had problems with our
cryostat and the techs that were working on this project had over a
century of experience with frozens.  Maybe Instrumedics could have come
to the rescue!

Beth Poole
Histo-Scientific Research Labs.
107 Killmon Road
P.O. Box 30
Basye, VA  22810

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