Dear Histonetter,

For those who have done Dystrophin stain(Immunoperoxidase)on muscle tissue.I 
need your help to solve my problem....

I use Ab from Novocastra to stain Dystrophin 1,2&3 with dilution
Dys 1 - 1:5 , Dys 2&3 - 1:10 for 1 hour at RT and secondary Ab I use
Rabbit Anti-mouse Immunoglobulin with dilution 1:100 for 1/2hr at RT.
(I use Tris buffer to dilute all Antibodies)
Then I develop with DAB for about 1-2 minutes,wash,counterstain with
Haematoxylin ,dehydrate ,clear and mount as usual.

The problem is fibrous tissue also stains up quite strongly(background 
staining)make us difficult to interpret/differentiate whether it's a 
positive staining of Dystrophin or background staining.

Could anyone of you helps me to solve this problem... please??
T.Q

FROM:
NORNAHAR KASSIM
MEDICAL LABORATORY TECHNOLOGIST
DEPT. OF PATHOLOGY
UNIVERSITY OF MALAYA
KUALA LUMPUR
MALAYSIA.


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