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From:Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
Date:Wed, 18 Aug 1999 09:47:55 +0000
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>Date: Tue, 17 Aug 1999 11:52:11 -0600
>From: Patsy.Ruegg@uchsc.edu
>Subject: DAB
>To: denisew2@juno.com
>Cc: histonet@pathology.swmed.edu
>Mime-Version: 1.0
	Any idea of e-mail or web site.

>I just wanted to plug this great DAB I have been using for the last few
>months.  It is liquid and kept in the freezer, but it does not freeze solid
>so you can pour it out for use each time.  Must have glycerine or something
>in it.  Anyway, it is good stuff, never percipitates, doesn't need filtering
>and is very clean and strong.  Another thing, it is pretty darn cheap.  It
>is made by Dave Brigatti's company Research Genetics 1-800-533-4363.  All of
>their other products I have used have been excellent as well.  I use their
>ready made pepsin digestion.  A DAKO rep recommended the DAB when I was
>having trouble with the DAKO DAB, must be good if one company recommends it
>over their own product, huh?  I do not work for Research Genetics or have
>any affiliation, just thought you all would like to know about some good
>stuff out there.
>Patsy Ruegg
>-----Original Message-----
>From: denise M m Long-Woodward [mailto:denisew2@juno.com]
>Sent: Thursday, August 12, 1999 11:00 AM
>To: JKotzuk@salud.unm.edu
>Cc: uvsgc@msu.oscs.montana.edu; histonet@pathology.swmed.edu
>Subject: Re: filtering DAB
>Dako Liquid DAB+ works great and the mixed solution can be saved for as
>long as two weeks(I don't save but some of my co-workers do)
>D Woodward, Boston, MA
>On Thu, 12 Aug 1999 11:55:53 -0600 "Joyce Kotzuk" <JKotzuk@salud.unm.edu>
>> I use syringe filters, I think they're regular glass fiber
>> non-sterile prefilters, and a 3 or 5 ml syringe. I don't know
>> exactly what we're filtering out, but the DAB turns clear after
>> filtering, whereas before it is slightly brown. Speaking of DAB,
>> I've been using Vector's DAB kit, and while it is easy and safe to
>> use, it has been browner than usual lately, and I'm beginning to
>> worry that it may be causing excessive background, so I'm thinking
>> of switching. Any recommendations for liquid DAB reagents? (I don't
>> want to be weighing out powder). Thanks, Joyce Kotzuk, UNM Pathology
>> >>> Gayle Callis <uvsgc@msu.oscs.montana.edu> 08/11/99 10:01AM >>>
>> Just curious, what are people using to filter DAB before applying to
>> tissue sections?  Microfilters, non sterile variety, for syringes?
>> or just a filter paper, # of filter paper please, ie Whatman # or
>> an equivalent.
>> this is for a very small amount of DAB, 2.5 mls frequently.
>> Gayle Callis
>Get the Internet just the way you want it.
>Free software, free e-mail, and free Internet access for a month!
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Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
G12 8QQ,
Tel: 0141 339 8855 Extn. 6602.
Fax: 0141 330 4100.
e-mail: ian.montgomery@bio.gla.ac.uk

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