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Date:Tue, 17 Aug 1999 11:52:11 -0600
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I just wanted to plug this great DAB I have been using for the last few
months.  It is liquid and kept in the freezer, but it does not freeze solid
so you can pour it out for use each time.  Must have glycerine or something
in it.  Anyway, it is good stuff, never percipitates, doesn't need filtering
and is very clean and strong.  Another thing, it is pretty darn cheap.  It
is made by Dave Brigatti's company Research Genetics 1-800-533-4363.  All of
their other products I have used have been excellent as well.  I use their
ready made pepsin digestion.  A DAKO rep recommended the DAB when I was
having trouble with the DAKO DAB, must be good if one company recommends it
over their own product, huh?  I do not work for Research Genetics or have
any affiliation, just thought you all would like to know about some good
stuff out there.
Patsy Ruegg
-----Original Message-----
From: denise M m Long-Woodward [mailto:denisew2@juno.com]
Sent: Thursday, August 12, 1999 11:00 AM
To: JKotzuk@salud.unm.edu
Cc: uvsgc@msu.oscs.montana.edu; histonet@pathology.swmed.edu
Subject: Re: filtering DAB

Dako Liquid DAB+ works great and the mixed solution can be saved for as
long as two weeks(I don't save but some of my co-workers do)
D Woodward, Boston, MA

On Thu, 12 Aug 1999 11:55:53 -0600 "Joyce Kotzuk" <JKotzuk@salud.unm.edu>
> I use syringe filters, I think they're regular glass fiber 
> non-sterile prefilters, and a 3 or 5 ml syringe. I don't know 
> exactly what we're filtering out, but the DAB turns clear after 
> filtering, whereas before it is slightly brown. Speaking of DAB, 
> I've been using Vector's DAB kit, and while it is easy and safe to 
> use, it has been browner than usual lately, and I'm beginning to 
> worry that it may be causing excessive background, so I'm thinking 
> of switching. Any recommendations for liquid DAB reagents? (I don't 
> want to be weighing out powder). Thanks, Joyce Kotzuk, UNM Pathology
> >>> Gayle Callis <uvsgc@msu.oscs.montana.edu> 08/11/99 10:01AM >>>
> Just curious, what are people using to filter DAB before applying to 
> tissue sections?  Microfilters, non sterile variety, for syringes? 
> or just a filter paper, # of filter paper please, ie Whatman # or 
> an equivalent. 
> this is for a very small amount of DAB, 2.5 mls frequently.  
> Gayle Callis

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