Controls for Immunofluorescence

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From:"Louri Caldwell" <louri_c@hotmail.com>
To:histonet@pathology.swmed.edu
Reply-To:
Date:Tue, 17 Aug 1999 18:47:18 PDT
Content-Type:text/plain; format=flowed

Hi Everyone,

The lab I am in is currently running low on positive control tissue for our 
IgG, IgA, IgM and C3 immunofluorescent assays.  If you do these studies, 
what type of tissue do you use as a positive control?  I had previously read 
where some labs use tonsil as a control for these studies, but one of the 
pathologists prefers to use skin (ie: bullous pemphigoid) or kidney(ie: 
glomerulonephritis). Would any of you happen to know of a vendor for such 
frozen tissue or acetone-fixed slides?

Any assistance is greatly appreciated.

Louri Caldwell

St. Mary's Hospital
Athens, Georgia


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