BrDU in mouse tissues
<< Previous Message | Next Message >>
From: | Shirley Chu <schu@deltagen.com> |
To: | Histonet@pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 18 Aug 1999 08:49:51 -0700 |
Content-Type: | text/plain; charset="us-ascii" ; format="flowed" |
To all histonetters,
I will be involved in a project in which I will be looking at cell
proliferation in the growth plate/bone development of mice using
BrDU. Some of the mice will be fairly young, anywhere from about 4-5
days old and the older ones about 20 days old. Primarily looking at
the femurs of these animals. Could anyone recommend to me an
antibody (or even better,supply me with a procedure) that they have
successfully used on mouse bones that are formalin fixed, paraffin
embedded. The bones for the younger mice will not be decalcified
prior to processing while the older ones will be (planning to use
formic acid for no longer than 2 days). Thanks in advance to all
responders.
Shirley Chu
Deltagen Inc.
San Carlos CA
<< Previous Message | Next Message >>