Acetone to fix india ink margins.
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From: | "Jeff Silverman" <peptolab@hamptons.com> |
To: | "RUSS ALLISON" <Allison@cardiff.ac.uk>, <histonet@pathology.swmed.edu> |
Reply-To: | |
Date: | Mon, 16 Aug 1999 10:33:24 -0400 |
Content-Type: | text/plain; charset=ISO-8859-1 |
We drop the specimens into acetone to fix the inked margins instantly and
neatly.
Jeff Silverman
----------
> From: RUSS ALLISON <Allison@cardiff.ac.uk>
> To: histonet@pathology.swmed.edu
> Subject: Re: Silver Nitrate for Margins -Reply
> Date: Monday, August 16, 1999 3:50 AM
>
> You can dry the Indian ink by touching with tissue/blotting paper;
> you do not have to leave the specimen sitting around to dry!
>
> Let me re-phrase my question "What does acetic acod/vinegar/Bouin's
> fluid do?
>
> Any of you ever spoken to an "ink scientist"? You would be VERY
> surprised how much there is to know about inks (Indian ink is a
> subject on its own)
>
> In the past, we have been interested in the particle size of Indian
> ink. Those guys not only know (and make inks of different particle
> size), they also vary the liquid in which the particles are
> suspended, so that the suspensoid has different characteristics,
> including drying time, spread, density, etc. They are useful friends
> in forensics also. We needed to assess the size of gaps between
> adjacent biological/non-biological structures and used variously
> sized Indian inks to do so. We sill do when looking at dental
> restorations.
>
> So back to my question - why vinegar, etc on inked margins? OK, I
> know why, but what is the rationale? Is it fixing the suspensoid
> phase of the ink? Is the suspensoid necessarily a protein?
>
> Still puzzled in Wales
> Russ Allison, Wales
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