Zenkers substitute -Reply

<< Previous Message | Next Message >>
From:DODI BORSAY <BORSAY.DODI@epamail.epa.gov>
To:kimberly.durland@Covance.Com, histonet@pathology.swmed.edu
Reply-To:
Date:Wed, 11 Aug 1999 12:42:20 -0400
Content-Type:text/plain

Kimberly,

We use a modified Zenker's fixative called Helly's:
     20,000 ml   Deionized Water
       1,000 gm   ZnCl2  (zinc chloride, CAS# 7646-85-7)
          500 gm   K2Cr2O7 (potassium dichromate, CAS# 7778-50-9)

Add 1 ml of 37% unbuffered formaldehyde (CAS# 000-050-000) per 
20 ml of Helly's solution prior to use.  This formulation replaces the
mercuric oxide component in the Zenker's with less toxic zinc chloride.
Still a 24 hour shelf life once formaldehyde is added.  

Dodi Borsay Horowitz
U.S. EPA
Atlantic Ecology Division
Narragansett, Rhode Island
borsay.dodi@epamail.epa.gov



<< Previous Message | Next Message >>