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From:Tom Kuwahara <tom@adpath.com>
To:HistoNet Server <HistoNet@pathology.swmed.edu>
Date:Fri, 06 Aug 1999 12:17:51 -0700
Content-Type:text/plain; charset=us-ascii

Dear histonetters:  Does anyone out there have a good protocol for
detecting beta galactosidase using X-Gal as a substrate?  Would like to
know specifically what dilution and which buffer you use in IHC on
frozens or at least a general range.  I have the X-GAL powder from
Molecular Probes.  Thanks, Tom
Thomas J. Kuwahara
Senior Immunohistochemist
Advanced Pathology Systems 
3801 Sacramento St. suite 621 
San Francisco, CA 94118 
415 750 6800 x23067 tel 
415 750 2332 fax 

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