Dear histonetters:  Does anyone out there have a good protocol for
detecting beta galactosidase using X-Gal as a substrate?  Would like to
know specifically what dilution and which buffer you use in IHC on
frozens or at least a general range.  I have the X-GAL powder from
Molecular Probes.  Thanks, Tom
-- 
******************************* 
Thomas J. Kuwahara
Senior Immunohistochemist
Advanced Pathology Systems 
3801 Sacramento St. suite 621 
San Francisco, CA 94118 
415 750 6800 x23067 tel 
415 750 2332 fax 
tom@adpath.com