Vector Red

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From:Katie B <>
To:Histonet Server <>
Date:Mon, 9 Aug 1999 05:21:58 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

I know that Vector Red is supposed to be non-soluble and can be
dehydrated and cleared, but...

I just finished up a staining round of BrdU using Vector Red
counterstained with Hematoxylin (Gill's #3) and remember seeing lots of
red on my positive controls (intestinal samples) without using a
microscope (this is normal for my protocol).  I then ran the slides up
through the alcohols and xylenes.  The first two racks looked great,
but my last rack of slides (which sat in the alcohols a little longer
than the others while I coverslipped) seemed faded and all the alcohols
and the first xylene were tinted strongly pink.  Anyone know what is
going on here?  

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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