I know that Vector Red is supposed to be non-soluble and can be
dehydrated and cleared, but...

I just finished up a staining round of BrdU using Vector Red
counterstained with Hematoxylin (Gill's #3) and remember seeing lots of
red on my positive controls (intestinal samples) without using a
microscope (this is normal for my protocol).  I then ran the slides up
through the alcohols and xylenes.  The first two racks looked great,
but my last rack of slides (which sat in the alcohols a little longer
than the others while I coverslipped) seemed faded and all the alcohols
and the first xylene were tinted strongly pink.  Anyone know what is
going on here?  



===
Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

e-mail: bresee98@yahoo.com
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