Tissue sections on slides
<< Previous Message | Next Message >>
From: | "Richard G. Lea" <rgl@rri.sari.ac.uk> |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 11 Aug 1999 12:46:45 +0100 |
Content-Type: | text/plain; charset=us-ascii |
To whoever can help,
I am attempting to carry out in-situ hybridisation on sections of tissue
which have been cultured for 24 hours. The control non-cultured tissues
are fine but after culture the tissue sections fall off the slides
during the in-situ protocol. We have tried TESPA and double coated
gelatin, PLL slides however the problem remains. How can I stop these
sections coming off the slides - any suggestions gratefully received.
Richard Lea
--
Richard G. Lea PhD
Senior Research Scientist
Division of Nutrition, Pregnancy and Development
Rowett Research Institiute
Greenburn Road
Bucksburn
Aberdeen AB21 9SB
Tel: 44(0) 1224 712751 x 2319
Fax: 44(0) 1224 716622
<< Previous Message | Next Message >>