Tissue sections on slides

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From:"Richard G. Lea" <rgl@rri.sari.ac.uk>
Date:Wed, 11 Aug 1999 12:46:45 +0100
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To whoever can help,

I am attempting to carry out in-situ hybridisation on sections of tissue
which have been cultured for 24 hours.  The control non-cultured tissues
are fine but after culture the tissue sections fall off the slides
during the in-situ protocol.  We have tried TESPA and double coated
gelatin, PLL slides however the problem remains.  How can I stop these
sections coming off the slides - any suggestions gratefully received.

Richard Lea
Richard G. Lea PhD
Senior Research Scientist
Division of Nutrition, Pregnancy and Development
Rowett Research Institiute
Greenburn Road
Aberdeen AB21 9SB

Tel:  44(0) 1224 712751 x 2319
Fax:  44(0) 1224 716622

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