Steiner on Helicobacter sp.

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From:Gayle Callis <>
Date:Wed, 11 Aug 1999 09:36:16 -0600

We opted for the Steiner for Helicobacter felis, another species used in
murine research.  Peggy Wenk had a lovely method to die for, and we use
it to stain 30 slides at a time, in a large waterbath.  She also used
microwave in her lab.  Her hints for Steiner were wonderful.  

Warthin Starry was just too lengthy to deal with, and very inconsistent
in our hands.  Steiner was simpler, kits are nice unless you have a huge
volume like mine.  Other methods were not specific enough to do what we
needed, Giemsa, etc.  

There is a wonderful immunostaining method published by Bert Dotson in
HistoLogic, would have been our choice for the H felis, but the antibody
did not cross react to this species.  Sadly, we would have done this
method instead of Steiner, and it is very specific! clean and beautiful
for H pylori.  You might want to consider immunostaining, in many ways,
nicer than dealing with disposal of silver or uranyl nitrate.  

Gayle Callis

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