Dear Histonetters,


We have been having problems using either saline coated slides or superfrost
plus slides for Bielschowsky Method for Neurofibrils.  The problem we are
experiencing is that we are getting a cloud of gray on the slides that will
not come off.  We tried using ammonium hydroxide to remove the precipitate,
but that does not work.  Using "untreated" slides doesn't work because the
tissue falls off during staining, even if the slides dry overnight or in the
oven.  By the way, our protocol is close to the AFIP protocol.  What does
everyone else do to rectifiy this problem?

Thanks,

Tom Galati
Histo-Scientific Research Labs.
(540)856-2222
histosci@shentel.net
107 Killmon Road
P.O. Box 30
Basye, VA  22810