To anyone out there who is using the Shandon Cadenza for 
immunostaining or a Shandon Rep:

We have 2 Cadenzas which we use everyday for our immunostaining and 
we have had these machines for a few years.  Recently, we started 
seeing a' bubble artifact' (areas of no staining) on the tissue. 
This is driving our pathologists crazy because there are times when 
the bubble is in the area they are interested in.  We just 
had both machines serviced and initially the staining seemed better 
but now the problem has returned.  We tried using a different wash 
buffer, but that made the problem worse.  If anyone can shed some 
light on this problem, we would really appreciate it.  

Thanks,
Michele Margiotta
Immunohistochemistry Lab
UH@Stony Brook,NY