Hi Jill,
On mouse tissue I use Pharmingen's CD31, stored at 4 C for at least a year
(not sure what they guarantee). I use Sigma's trypsin tablets (T-7168),
just add 1ml of water and dissolve. Digest tissues with trypsin solution at
37 C for 10 minutes. This works great for me!

Marcia

At 04:32 PM 8/11/99 +0100, Jill McVee wrote:
>To all you wonderful histonetters,
>                                       A student of mine is having problems
>with pecam-1 (CD31) she stained some slides about six months ago and all
>the blood vessel were positive. She then store the antibody at -20oc  as
>instructed but this last experiment seems to be very patchy in the reaction
>sites. Can anyone suggest a foolproof antigen retrival method for this
>antibody, taking into consideration that they were fixed in 4%
>Paraformaldehyde and processed to wax.
>Or maybe give me an indication of the shelf(freezer) life for this ab.
>Gratefully yours,...... Jill......
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