Dako Liquid DAB+ works great and the mixed solution can be saved for as
long as two weeks(I don't save but some of my co-workers do)
D Woodward, Boston, MA


On Thu, 12 Aug 1999 11:55:53 -0600 "Joyce Kotzuk" <JKotzuk@salud.unm.edu>
writes:
> I use syringe filters, I think they're regular glass fiber 
> non-sterile prefilters, and a 3 or 5 ml syringe. I don't know 
> exactly what we're filtering out, but the DAB turns clear after 
> filtering, whereas before it is slightly brown. Speaking of DAB, 
> I've been using Vector's DAB kit, and while it is easy and safe to 
> use, it has been browner than usual lately, and I'm beginning to 
> worry that it may be causing excessive background, so I'm thinking 
> of switching. Any recommendations for liquid DAB reagents? (I don't 
> want to be weighing out powder). Thanks, Joyce Kotzuk, UNM Pathology
> 
> >>> Gayle Callis <uvsgc@msu.oscs.montana.edu> 08/11/99 10:01AM >>>
> Just curious, what are people using to filter DAB before applying to 
> 
> tissue sections?  Microfilters, non sterile variety, for syringes? 
> or just a filter paper, # of filter paper please, ie Whatman # or 
> an equivalent. 
> 
> this is for a very small amount of DAB, 2.5 mls frequently.  
> 
> Gayle Callis
> 
> 
> 

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