Re: eosin fading

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From:FreidaC@aol.com
To:uvsgc@msu.oscs.montana.edu, histonet@pathology.swmed.edu
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Date:Thu, 5 Aug 1999 18:59:21 EDT
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Thanks Gayle:

I have been talking to this lab for months it seems about fading eosin, and 
everything that I have suggested does not seem to correct the problem, so I 
am going there next week and try to see if I can solve the problem.  I have 
even had them pHing the water to be sure that it is not real alkaline, and 
adding acetic acid to the alcohol before the eosin to be sure that the pH is 
correct, but nothing seems to help.  I hope that an on site visit will bring 
the problem to light.  Just thought I would see if anyone else is currently 
experiencing the problem. They are using automated staining.

Freida



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