Re: Tissue sections on slides
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From: | "P. Emry" <emry@u.washington.edu> |
To: | "John C. Dennis" <dennijc@vetmed.auburn.edu> |
Reply-To: | |
Date: | Wed, 11 Aug 1999 14:31:10 -0700 (PDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
Hi Netters
I am having the problems of tissue coming off the slides durning Brdu
staining. I am not the one doing the staining, but I'm the one cutting
the sections. Is there a etoh step in Brdu that can be avoided so that
the tissue will stay on? Any other modification that will help?
I ordered my silane from Sigma then saw that Surgipath only makes it up
when you order it because it has a shelf life of 3 months. Any thoughts
on this or experience?
Trisha
On Wed, 11 Aug 1999, John C. Dennis wrote:
> Richard
>
> Are you using any EtOH in treating your tissue before you mount the
> sections on slides?
> We were having a similar problem just the other week. Our frozen
> sections were coming off the
> slides poly L lysine or no. The tissue had been fixed but held in 70%
> EtOH until sectioning.
> Once I stopped exposing the tissues to EtOH the sections stopped coming
> off the slides.
>
> John Carroll Dennis
> Anatomy, Physiology, and Pharmacology
> 109 Greene Hall
> Auburn University, AL 36849
>
>
> On Wed, 11 Aug 1999, Richard G. Lea wrote:
>
> > To whoever can help,
> >
> > I am attempting to carry out in-situ hybridisation on sections of tissue
> > which have been cultured for 24 hours. The control non-cultured tissues
> > are fine but after culture the tissue sections fall off the slides
> > during the in-situ protocol. We have tried TESPA and double coated
> > gelatin, PLL slides however the problem remains. How can I stop these
> > sections coming off the slides - any suggestions gratefully received.
> >
> > Richard Lea
> > --
> > Richard G. Lea PhD
> > Senior Research Scientist
> > Division of Nutrition, Pregnancy and Development
> > Rowett Research Institiute
> > Greenburn Road
> > Bucksburn
> > Aberdeen AB21 9SB
> >
> > Tel: 44(0) 1224 712751 x 2319
> > Fax: 44(0) 1224 716622
> >
>
>
>
Trisha
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