Re: Tissue sections on slides

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From:"P. Emry" <emry@u.washington.edu>
To:"John C. Dennis" <dennijc@vetmed.auburn.edu>
Reply-To:
Date:Wed, 11 Aug 1999 14:31:10 -0700 (PDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

Hi Netters

I am having the problems of tissue coming off the slides durning Brdu
staining.  I am not the one doing the staining, but I'm the one cutting
the sections.  Is there a etoh step in Brdu that can be avoided so that
the tissue will stay on?  Any other modification that will help?

I ordered my silane from Sigma then saw that Surgipath only makes it up
when you order it because it has a shelf life of 3 months.  Any thoughts
on this or experience?

Trisha   
On Wed, 11 Aug 1999, John C. Dennis wrote:

> Richard
> 
> Are you using any EtOH in treating your tissue before you mount the
> sections on slides?  
> We were having a similar problem just the other week.  Our frozen
> sections were coming off the
> slides poly L lysine or no.  The tissue had been fixed but held in 70%
> EtOH until sectioning.
> Once I stopped exposing the tissues to EtOH the sections stopped coming
> off the slides.
> 
> John Carroll Dennis
> Anatomy, Physiology, and Pharmacology
> 109 Greene Hall
> Auburn University, AL  36849
> 
> 
> On Wed, 11 Aug 1999, Richard G. Lea wrote:
> 
> > To whoever can help,
> > 
> > I am attempting to carry out in-situ hybridisation on sections of tissue
> > which have been cultured for 24 hours.  The control non-cultured tissues
> > are fine but after culture the tissue sections fall off the slides
> > during the in-situ protocol.  We have tried TESPA and double coated
> > gelatin, PLL slides however the problem remains.  How can I stop these
> > sections coming off the slides - any suggestions gratefully received.
> > 
> > Richard Lea
> > -- 
> > Richard G. Lea PhD
> > Senior Research Scientist
> > Division of Nutrition, Pregnancy and Development
> > Rowett Research Institiute
> > Greenburn Road
> > Bucksburn
> > Aberdeen AB21 9SB
> > 
> > Tel:  44(0) 1224 712751 x 2319
> > Fax:  44(0) 1224 716622
> > 
> 
> 
> 

Trisha  




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