Re: Paraffin embedding and immunocytochemistry

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From:"Tony Henwood" <>
Date:Fri, 6 Aug 1999 19:14:18 +0000
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> John,
> Simple question you raised.  We are working on the answer - not easy 
> but very interesting!
> If the heat of molten paraffin "destroys" immuno-reactivity, how come 
> we use heat for antigen retrieval?
> Russ Allison, Wales

Dear Russ,
I suggest that the heat that is used for antigen retrieval is "wet 
heat" not ?dry heat as would possibly be occuring during processing 
and slide drying.

Does this sound good or should I go back to bottle of scotch :)

Regards,  Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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