Re: Paraffin Problems
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From: | "R.Wadley" <s9803537@pop3.unsw.edu.au> |
To: | HistoNet@pathology.swmed.edu |
Reply-To: | |
Date: | Wed, 11 Aug 1999 11:08:46 +1000 |
Content-Type: | text/plain; charset="us-ascii" |
Dear Sue,
From what you've described:
Wax is not the problem even if there was a bad batch, you changed brands.
Xylene, distilled or otherwise is not the problem.
The processor reagents in general are not a problem.
However, the problem does hint at incomplete infiltration. Have any of
the settings on the processor changed, ie, stirrer is off, pressure/vacuum
cycles been altered or turned off? Is there a problem with the machine
that the diagnostics are not detecting?
The other point is, has anybody checked the waterbath temp, is it hotter
than normal?
Regards
Rob W.
At 08:26 AM 8/10/99 -0700, you wrote:
>I hope someone can help us.
>For the past month our paraffin surrounding the tissue has been blowing
>up in the water bath. We have two processors and the problem occured
>in both machine. The paraffin we used was Tissue Prep. We also use
>distill Xylene. These are the steps we took.
>1. Switched to Surgipath infiltrating and embedding paraffin. This
>step didn't solve our problem.
>2. We put fresh Xylene into both stations for all processors. We
>still have the problem.
>3. We changed all solutions (formalin to paraffin) and the problem
>still is happening.
>If anyone has any suggestions please respond.
R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) +61 (2) 9385 3517
Ph (AH) +61 (2) 9555 1239
Fax +61 (2) 9385 1591
E-mail r.wadley@unsw.edu.au
www http://www.micro.unsw.edu.au/caf.html
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