Re: Paraffin Problems

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From:"R.Wadley" <>
Date:Wed, 11 Aug 1999 11:08:46 +1000
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	Dear Sue,

	From what you've described:
	Wax is not the problem even if there was a bad batch, you changed brands.
	Xylene, distilled or otherwise is not the problem.
	The processor reagents in general are not a problem.

	However, the problem does hint at incomplete infiltration.  Have any of
the settings on the processor changed, ie, stirrer is off, pressure/vacuum
cycles been altered or turned off?  Is there a problem with the machine
that the diagnostics are not detecting?

	The other point is, has anybody checked the waterbath temp, is it hotter
than normal?


	Rob W.

At 08:26 AM 8/10/99 -0700, you wrote:
>I hope someone can help us.
>For the past month our paraffin surrounding the tissue has been blowing
>up in the water bath.  We have two processors and the problem occured
>in both machine.  The paraffin we used was Tissue Prep.  We also use
>distill Xylene.  These are the steps we took.
>1.  Switched to Surgipath infiltrating and embedding paraffin.  This
>step didn't solve our problem.
>2.  We put fresh Xylene into both stations for all processors.  We
>still have the problem.
>3.  We changed all solutions (formalin to paraffin) and the problem
>still is happening.
>If anyone has any suggestions please respond.

R. Wadley, B.App.Sc. M.L.S, Grad.Dip.Sc.MM
Laboratory Manager
Cellular Analysis Facility
School of Microbiology & Immunology
UNSW, New South Wales, Australia, 2052
Ph (BH) 	+61 (2) 9385 3517
Ph (AH)	+61 (2) 9555 1239
Fax 	+61 (2) 9385 1591

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