I have occasionally done this.  It is a relatively well established method
of demonstrating lipofuscins, particularly the more fatty kind.  Yes, they
do survive treatment with xylene in paraffin processing.

I use the method with oil red O dissolved in propylene glycol and stain for
at least an hour.  You MUST mount with an aqueous mountant though, or the
dye is extracted.

Bryan Llewellyn



----- Original Message -----
From: Histo-Scientific Research Laboratory <histosci@shentel.net>
To: Linda McGraf <HistoNet@pathology.swmed.edu>
Sent: August 6, 1999 6:16 AM
Subject: Paraffin Oil Red O staining


> Dear Histonetters,
>
> Has anyone ever done an Oil Red O stain on paraffin embedded tissues?  I
am
> wondering why anyone would do frozens on fatty tissues if the lipids
stayed
> in tact when they where processed, and paraffin embedded?  I would love to
> know your results if you have done this procedure.
>
> Thanks,
>
> Beth Poole
> Histo-Scientific Research Labs.
> (540)856-2222
> histosci@shentel.net
> P.O. Box 30
> 107 Killmon Road
> Basye, VA  22810
>
>
>