Roy,

There are a number of commercially available products out there for detecting 
mycolplasma contamination.  For instance, Molecular Probes sells the MycoFluor 
Mycoplasma Detection Kit.  I have no idea how this compares to other mycoplasma 
detection kits on the market.  If you are experienced, then all you really need is 
a cell-permeable fluorescent DNA dye to detect these intracellular cell 
contaminants.  The advantage to the kits is that they provide some sort of 
controls, so if you are inexperienced, there's less ambiguity in interpreting the 
results.  You might do a net search for other products on the market.

Karen in Oregon
    



Date:          Wed, 4 Aug 1999 14:27:46 +0930
From:          "Roy Ellis" <roy.ellis@imvs.sa.gov.au>
Subject:       Mycoplasma
To:            "Histonet" <HISTONET@pathology.swmed.edu>

I have been asked to post the following:
Does anyone know of a quick, user-friendly technique to demonstrate
mycoplasma sp in cells from tissue culture preparations (Caco 2).
Roy Ellis
roy.ellis@imvs.sa.gov.au