Re: Micro-chatters in gastric biopsies

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From:V Hurst <>
To:"Hall, Phil" <>
Date:Wed, 04 Aug 1999 20:22:02 -0400
Content-Type:text/plain; charset=us-ascii

We recently had this problem which seems to have been rectified by letting the
block rehydrate  on ice between levels, at least we have had no more complaints.

V Hurst MLT
Sudbury ON

"Hall, Phil" wrote:

> Does anyone see microscopic chatters (cracks parallel to the knife edge) in
> endoscopy biopsies (approx 0.2cm diameter)?
> We have been seeing these in our paraffin sections of these biopsies so
> badly that the Pathologists sometimes complain it makes diagnosis difficult.
> We do not seem to have any problems with large tissue blocks or bone.  We
> cut sections at 3 microns using a manual rotary microtome with disposable
> S35 blades.
> So far we have done the following to try to stop this happening:
> changed microtome - including buying a new one
> changed blades - including using a different supplier
> changed the microtome knife angle
> cut them slower and faster
> cut them very cold or warm
> let them float on the water bath for longer or picked them up straight away
> Any other ideas?
> Phil Hall
> Paediatric Pathology
> St Michael's Hospital
> UK

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