Re: Fixatives
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From: | Ken Turner <ken.turner@stonebow.otago.ac.nz> |
To: | "Lisa Dillard-Telm" <ldillardtelm@primate.ucdavis.edu> |
Reply-To: | |
Date: | Wed, 4 Aug 1999 08:53:01 +1200 |
Content-Type: | text/plain; charset="us-ascii" |
>Hello everyone,
>
> I've been asked to fix late stage embryos for unknown future
>immunohistochemical applications (paraffin embedded). Does anyone have a
>preferred fixative that I can try that would work for most
>immunohistochemical applications?
>
>Thank you,
>Lisa.
Hi Lisa,
We have successfully fixed embryonic material for immunohistology for a
number of our clients by microwave assisting formalin fixation. Depending
on the size of the tissue we immerse in 10% buffered formalin for 1 to 4
hours then microwave (using a probe) to 50oC for 2 to 5 minutes. On cooling
(5min) we transfer to 70% ethyl alcohol to await processing. The reduced
time in formalin retains tissue antigenicity whilst acheiving full
fixation. Good luck.
Ken.
>
>
>
>Lisa Dillard-Telm
>Staff Research Associate
>California Regional Primate Research Center
>Davis, California 95616
>(530)752-4837
>ldillard@ucdavis.edu
Kenneth W Turner
Senior Teaching Fellow
Unit Manager / Pathology Histology Unit
Department of Pathology
Otago Medical School
P.O.Box 913
Dunedin
New Zealand
Tel. 03 4797135 International 0064 3 4797135
Fax. 03 4797136 International 0064 3 4797136
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