Re: Fixatives

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From:Ken Turner <>
To:"Lisa Dillard-Telm" <>
Date:Wed, 4 Aug 1999 08:53:01 +1200
Content-Type:text/plain; charset="us-ascii"

>Hello everyone,
>     I've been asked to fix late stage embryos for unknown future
>immunohistochemical applications (paraffin embedded).  Does anyone have a
>preferred fixative that I can try that would work for most
>immunohistochemical applications?
>Thank you,

Hi Lisa,

We have successfully fixed embryonic material for immunohistology for a
number of our clients by microwave assisting formalin fixation. Depending
on the size of the tissue we immerse in 10% buffered formalin for 1 to 4
hours then microwave (using a probe) to 50oC for 2 to 5 minutes. On cooling
(5min) we transfer to 70% ethyl alcohol to await processing. The reduced
time in formalin retains tissue antigenicity whilst acheiving full
fixation. Good luck.

>Lisa Dillard-Telm
>Staff Research Associate
>California Regional Primate Research Center
>Davis, California  95616

Kenneth W Turner
Senior Teaching Fellow
Unit Manager / Pathology Histology Unit
Department of Pathology
Otago Medical School
P.O.Box 913
New Zealand

Tel. 03 4797135  International  0064 3 4797135
Fax. 03 4797136  International  0064 3 4797136

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