Re: Fading of eosin

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Date:Wed, 4 Aug 1999 16:01:03 -0700
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I did see this at one point. It turned out that the tech was rinsing in water
instead of going straight into 95% alcohol from the eosin. (We use an alcohol
based Eosin-Y.) on 08/04/99 01:22:23 PM

cc:    (bcc: Lori Miller/WLGORE)
Subject:  Fading of eosin

Have any of you in histoland had a problem with pale or fading eosin in the
H&E stain when you have changed nothing in your procedure.  If so, and you
solved the problem - what was the cause?


Freida Carson

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