Jill,

I trust that the -20C was not a frost free unit!!!

We use CD31(clone JC-50A) at 1:50 o/n @ RT, following pronase digestion.
No problems for the past 3 years!

Regards

Bryan

>----------
>From: 	jm36@st-andrews.ac.uk[SMTP:jm36@st-andrews.ac.uk]
>Sent: 	August 11, 1999 10:32 AM
>To: 	histonet@pathology.swmed.edu
>Subject: 	pecam-1 or CD31
>
>To all you wonderful histonetters,
>                                       A student of mine is having problems
>with pecam-1 (CD31) she stained some slides about six months ago and all
>the blood vessel were positive. She then store the antibody at -20oc  as
>instructed but this last experiment seems to be very patchy in the reaction
>sites. Can anyone suggest a foolproof antigen retrival method for this
>antibody, taking into consideration that they were fixed in 4%
>Paraformaldehyde and processed to wax.
>Or maybe give me an indication of the shelf(freezer) life for this ab.
>Gratefully yours,...... Jill......
>
>
>
>