RE: pecam-1 or CD31

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From:"Hewlett Bryan (CMH)" <HEWLETT@EXCHANGE1.CMH.ON.CA>
To:"''" <>, "''" <>
Date:Wed, 11 Aug 1999 13:43:17 -0400
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I trust that the -20C was not a frost free unit!!!

We use CD31(clone JC-50A) at 1:50 o/n @ RT, following pronase digestion.
No problems for the past 3 years!



>Sent: 	August 11, 1999 10:32 AM
>Subject: 	pecam-1 or CD31
>To all you wonderful histonetters,
>                                       A student of mine is having problems
>with pecam-1 (CD31) she stained some slides about six months ago and all
>the blood vessel were positive. She then store the antibody at -20oc  as
>instructed but this last experiment seems to be very patchy in the reaction
>sites. Can anyone suggest a foolproof antigen retrival method for this
>antibody, taking into consideration that they were fixed in 4%
>Paraformaldehyde and processed to wax.
>Or maybe give me an indication of the shelf(freezer) life for this ab.
>Gratefully yours,...... Jill......

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