RE: paraffin embedding

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From:Brown Alexander <>
To:HistoNet <>
Date:Fri, 13 Aug 1999 10:04:00 +0100
Content-Type:text/plain; charset="iso-8859-1"

Hi Edna,
	The variety of procedures/techniques/tribulations associated
with paraffin processing and embedding are too vast for an E-mail
tutorial. My advice would be to either speak to your Shandon rep or
preferably, seek out a local hospital and speak nicely to the people in
the lab. See if you can spend a day with them.
	Best of luck,
		Alex Brown
		Crosshouse Hospital
		Kilmarnock, Scotland.
P.S. If you can get the funding you could pop over here :)

From: Edna_J_Gonzalez/
Subject: paraffin embedding
Date: Thursday, August 12, 1999 8:01PM

I have experience with working with cryostat ,but not a lot working with
paraffin embedding. Recently, we changed to paraffin embedding in the
Can someone help me and guide me to do paraffin embedding? What do I
to do to prepare the tissue cassettes, how is this done? I have the
but I want to be sure. How do you use the embedding rings? Is there a
book that will show me how to do paraffin embedding step-by-step?

We are going to use a Citadel tissue processor (from Shandon) and I have
never used it before either. Hope someone can help me on this.

Edna J. Gonzalez
Histology Lab
PowderJect Vaccines

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