RE: paraffin embedding

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From:Brown Alexander <AlexB@nayrshire.scot.nhs.uk>
To:HistoNet <HistoNet@pathology.swmed.edu>
Reply-To:
Date:Fri, 13 Aug 1999 10:04:00 +0100
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Hi Edna,
	The variety of procedures/techniques/tribulations associated
with paraffin processing and embedding are too vast for an E-mail
tutorial. My advice would be to either speak to your Shandon rep or
preferably, seek out a local hospital and speak nicely to the people in
the lab. See if you can spend a day with them.
	Best of luck,
		Alex Brown
		Crosshouse Hospital
		Kilmarnock, Scotland.
P.S. If you can get the funding you could pop over here :)

 ----------
From: Edna_J_Gonzalez/Powderject@powderject.com
To: histonet@pathology.swmed.edu
Subject: paraffin embedding
Date: Thursday, August 12, 1999 8:01PM



I have experience with working with cryostat ,but not a lot working with
paraffin embedding. Recently, we changed to paraffin embedding in the
lab.
Can someone help me and guide me to do paraffin embedding? What do I
have
to do to prepare the tissue cassettes, how is this done? I have the
concept
but I want to be sure. How do you use the embedding rings? Is there a
good
book that will show me how to do paraffin embedding step-by-step?

We are going to use a Citadel tissue processor (from Shandon) and I have
never used it before either. Hope someone can help me on this.

Edna J. Gonzalez
Histology Lab
PowderJect Vaccines





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