We had this problem with the "bleeding" eosin when we were using a mounting
medium from Richard Allen.  We are using their Clear-Rite but the eosin
bled.  We had investigated everything else first (alcohol etc.).  As soon as
we changed our mounting medium the problem was gone.  We are now using
Permount.

Jennifer MacDonald
San Antonio Community Hospital
Upland, CA

> ----------
> From: 	kkdulany@UNMC.EDU[SMTP:kkdulany@UNMC.EDU]
> Sent: 	Friday, August 06, 1999 8:18 AM
> To: 	FreidaC@aol.com
> Cc: 	uvsgc@msu.oscs.montana.edu; histonet@pathology.swmed.edu
> Subject: 	Re: eosin fading
> 
> Dear Frieda,
> This is a weird coincidence.  I had never heard of eosin fading until a
> couple of days
> ago.
> 
> Then I did a batch of slides (ours are done by hand) and the investigator
> who they were
> for was on vacation so I did not deliver them that day.  I looked at them
> a few days later
> and the eosin was bleeding out into the surrounding area, outside the
> tissue itself.  Is
> this what you meant?
> I will check the alcohols as I feel maybe these are not true 95 or 100 per
> cent due to our
> very humid weather last week and maybe enough eosin was not taking out in
> those
> dehydration steps.
> Karen Dulany HTL (ASCP)
> Omaha, NE
> 
> 
>