RE: Vector Red

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From:Katie B <>
To:Histonet Server <>
Date:Tue, 10 Aug 1999 05:46:36 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

Called Vector and viola!  Yep, Vector Red will be removed with
excessive exposure to alcohols or xylenes, 30 min max to be exact.  So
I guess I better get faster at my coverslipping or else work in smaller
batches!  So glad they include this kind of information on the Spec
sheet.  (Gee... can you smell the sarcasm?)

> I know that Vector Red is supposed to be non-soluble and can be
> dehydrated and cleared, but...
> I just finished up a staining round of BrdU using Vector Red
> counterstained with Hematoxylin (Gill's #3) and remember seeing
> lots of
> red on my positive controls (intestinal samples) without using a
> microscope (this is normal for my protocol).  I then ran the
> slides up
> through the alcohols and xylenes.  The first two racks looked
> great,
> but my last rack of slides (which sat in the alcohols a little
> longer
> than the others while I coverslipped) seemed faded and all the
> alcohols
> and the first xylene were tinted strongly pink.  Anyone know what
> is
> going on here?

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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