RE: Vector Red

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From:"Bruce W. Brodersen" <>
To:"Katie B" <>, "Histonet Server" <>
Date:Mon, 9 Aug 1999 16:13:05 -0500
Content-Type:text/plain; charset="iso-8859-1"

I've seem something similar to this.  In these cases, there is a
lot of background or nonspecific adherance of stain (Vector Red,
due to I don't know what, perhaps bad washing for whatever
reason, pH of wash buffers?) to the glass that comes of during
dehydration.  In my experience, it usually doesn't affect the
specific staining.

Bruce W. Brodersen, DVM, PhD   Email:
University of Nebraska         Voice:  402 472-1434
Veterinary Diagnostic Center   FAX:    402 472-3094
Lincoln, NE  68583-0907

-----Original Message-----
From: Katie B []
Sent: Monday, August 09, 1999 3:05 PM
To: Histonet Server
Subject: Vector Red

I know that Vector Red is supposed to be non-soluble and can be
dehydrated and cleared, but...

I just finished up a staining round of BrdU using Vector Red
counterstained with Hematoxylin (Gill's #3) and remember seeing
lots of
red on my positive controls (intestinal samples) without using a
microscope (this is normal for my protocol).  I then ran the
slides up
through the alcohols and xylenes.  The first two racks looked
but my last rack of slides (which sat in the alcohols a little
than the others while I coverslipped) seemed faded and all the
and the first xylene were tinted strongly pink.  Anyone know what
going on here?

Catherine "Katie" Bresee Bennett
Laboratory for Experimental Pathology
Department of Veterinary Pathology
Michigan State University

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