RE: Silver Staining problem

<< Previous Message | Next Message >>
From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:histoNet@pathology.swmed.edu, "'Histo-Scientific Research Laboratory'" <histosci@shentel.net>
Reply-To:
Date:Mon, 9 Aug 1999 17:59:45 -0400
Content-Type:text/plain

Sta-On tissue Section Adhesive(Surgipath #03105) in the water bath - works
for me. Better than the Masson gelatin or Mayer's albumin. Place slides in
oven overnight, and stain. I have lost very few sections with this product,
except for the occasional insufficiently processed specimens. Use it for all
Specials and IHC as well.

Surgipath 800-226-3035


Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center

> ----------
> From: 	Histo-Scientific Research
> Laboratory[SMTP:histosci@shentel.net]
> Sent: 	Monday, August 09, 1999 10:49 AM
> To: 	histoNet@pathology.swmed.edu
> Subject: 	Silver Staining problem
> 
>     Dear Histonetters,
> 
> 
> We have been having problems using either saline coated slides or
> superfrost
> plus slides for Bielschowsky Method for Neurofibrils.  The problem we are
> experiencing is that we are getting a cloud of gray on the slides that
> will
> not come off.  We tried using ammonium hydroxide to remove the
> precipitate,
> but that does not work.  Using "untreated" slides doesn't work because the
> tissue falls off during staining, even if the slides dry overnight or in
> the
> oven.  By the way, our protocol is close to the AFIP protocol.  What does
> everyone else do to rectifiy this problem?
> 
> Thanks,
> 
> Tom Galati
> Histo-Scientific Research Labs.
> (540)856-2222
> histosci@shentel.net
> 107 Killmon Road
> P.O. Box 30
> Basye, VA  22810
> 
> 



<< Previous Message | Next Message >>