RE: Silver Staining problem
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> |
To: | histoNet@pathology.swmed.edu, "'Histo-Scientific Research Laboratory'" <histosci@shentel.net> |
Reply-To: | |
Date: | Mon, 9 Aug 1999 17:59:45 -0400 |
Content-Type: | text/plain |
Sta-On tissue Section Adhesive(Surgipath #03105) in the water bath - works
for me. Better than the Masson gelatin or Mayer's albumin. Place slides in
oven overnight, and stain. I have lost very few sections with this product,
except for the occasional insufficiently processed specimens. Use it for all
Specials and IHC as well.
Surgipath 800-226-3035
Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
> ----------
> From: Histo-Scientific Research
> Laboratory[SMTP:histosci@shentel.net]
> Sent: Monday, August 09, 1999 10:49 AM
> To: histoNet@pathology.swmed.edu
> Subject: Silver Staining problem
>
> Dear Histonetters,
>
>
> We have been having problems using either saline coated slides or
> superfrost
> plus slides for Bielschowsky Method for Neurofibrils. The problem we are
> experiencing is that we are getting a cloud of gray on the slides that
> will
> not come off. We tried using ammonium hydroxide to remove the
> precipitate,
> but that does not work. Using "untreated" slides doesn't work because the
> tissue falls off during staining, even if the slides dry overnight or in
> the
> oven. By the way, our protocol is close to the AFIP protocol. What does
> everyone else do to rectifiy this problem?
>
> Thanks,
>
> Tom Galati
> Histo-Scientific Research Labs.
> (540)856-2222
> histosci@shentel.net
> 107 Killmon Road
> P.O. Box 30
> Basye, VA 22810
>
>
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