RE: Signal vs. Intact Tissue

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From:"Nocito, Joseph" <>
To:"'Matthew Ogdie'" <>,
Date:Thu, 12 Aug 1999 15:56:47 -0500

you didn't say how long you keep the slides in a drying oven.  We were
having problems with bone marrows (even using positively charged
slides)until we heated the slides overnight at 60 degrees C.  We had better
tissue adhesion.  If that fails, Surgipath has their own brand of charged
slides and Biocare has their Clingon slides.  Good luck

Joe Nocito, B.S., HT(ASCP)QIHC
Histology Supervisor
Christus Santa Rosa Hospitals
San Antonio, Texas 

> -----Original Message-----
> From:	Matthew Ogdie []
> Sent:	Thursday, August 12, 1999 10:58 AM
> To:
> Subject:	Signal vs. Intact Tissue
> Hello,
> Someone I know is having major issues and I need your help. The problem is
> as follows:
> She is running  IHC on paraffin embedded tissue(mouse/human xenograph),
> Rabbit polyclonal VEGF.
> The assay was working fine three months ago with the same reagents and the
> exact same tissue. At the time she used Citra Ag retrieval for 5 min., and
> proceeded with basic IHC.
> Now, the only way she can get a signal is by using Tris/EDTA(pH10)
> pretreatment boil/simmer/steam(she's tried it all) for 10-15 min. This is
> okay for small sections, but she needs to do larger sections which
> encompass
> multiple organs(kidney,intestine,spleen). These larger sections come right
> off the slide. Thus, she is stuck between no signal and the tissue coming
> off the slide. She has used positive slides, silane adhesive...all of the
> usual tricks. I have asked everyone else I know and have run the full
> gambit
> of tricks.
> Help,
> Matthew

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