Movat's Modified Pentachrome uses a 2.0% alcian blue in 1.0% aqueous acetic
acid for 10 minutes, staining all acid mucins (chondroitin sulphate A,
chondroitin sulphate B, chondroitin sulphate C, heparin sulphate, keratin
sulphate, hyaluronosulphate) - blue.


Eric C. Kellar 
Histology/Immunohistochemistry
University of Pittsburgh Medical Center

	----------
	From:  Mark Elliott [SMTP:MElliott@mrl.ubc.ca]
	Sent:  Friday, August 06, 1999 11:08 AM
	To:  histonet@pathology.swmed.edu
	Subject:  Movat's pentachrome 

	We have a researcher here who is looking at Movat's pentachrome
stained 
	slides.  They are wanting to know what polysaccharides are stained
by this 
	method-is it all of them, or just a subset?  The hospital lab does
the 
	staining for us and we can't find a reference to the method in any
of our 
	textbooks.  Does anyone know the answer, or can they point me to 
	reference?  Any help would be greatly appreciated.
	Thanks in advance.

	Mark


	Mark Elliott, PhD
	Research Associate
	UBC-Pulmonary Research Lab
	St. Paul's Hospital
	Vancouver, BC Canada V6Z 1Y6
	604-631-5351 (FAX)