Alizarin red S (sodium alizarin sulfonate) method is a reliable stain for
calcium salts and or crystals. Calcium deposits become birefringent with a
2% aqueous alizarin red S, adjusted with 10% ammonium hydroxide to a pH of
4.2. End point staining must controlled microscopically when the calcium
deposits become reddish-orange. A good control would be kidney
hypercalcinosis or breast calcification. I would be glad to fax you a copy
of the procedure by McKee-Russell, if this is the method your pathologist is
requesting.



Eric C. Kellar
University of Pittsburgh Medical Center

-----Original Message-----
From: South Path 2 Whicoot
To: histonet@pathology.swmed.edu
Sent: 8/13/99 1:12 PM
Subject: Luzerin Red

Hello everyone!

One of our pathologists asked us for a Luzerin Red stain for crystals.
Does anyone have any information on this?  Thanks to all in advance.


Southpath2
St. Joseph Medical Center
Towson, Md. 21204


_______________________________________________________________
Get Free Email and Do More On The Web. Visit http://www.msn.com