RE: LR White immuno

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From:Tamara Howard <>, Histology listserver <>
Date:Fri, 13 Aug 1999 17:04:42 -0400 (EDT)
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Any chance that the sections are large enough (=visible)  that you could
do a floating immuno? Use a wire loop to transfer them from drop to drop
of your solutions, or work in a multi-well (TC-type) plate? Then stick
them down to slides after all the real work is done....I've done really
small samples this way (sections and tissue bits) and the only catch is
that you have to be really careful changing solutions. Vacuum aspiration
is out!

Tamara Howard

>Dear colleagues,
>I would like to do immunohistochemistry on 1 micron LR white sections
>before doing it on thin sections for EM.
>1)      How can I prevent sections from wrinkling? I don't want to use
>heat to preserve antigenicity.
>2)      Without using heat how can I attach them to slides so that they
>do not fall off during the immuno. I have already tried to air-dry them
>on Superfrost slides but they easily fall off after few washes.
>Thanking you in advance for your help,

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