RE: Golgi-Cox dark areas.
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From: | "Kellar, Eric" <kellarec@MSX.UPMC.EDU> |
To: | HistoNet@pathology.swmed.edu, "'Bruce A Rasmussen'" <brasmuss@osf1.gmu.edu> |
Reply-To: | |
Date: | Thu, 12 Aug 1999 11:20:11 -0400 |
Content-Type: | text/plain |
Pugh et al 1993 described the destaining of sections blackened during and
after the Golgi-Cox method.
The method is as follows:
1. Bring slides to water.
2. Place slides into Lugol's Iodine for 5 minutes.
3. Wash slides in running tap for 1 min.
4. Place slides into 5% sodium thiosulphate for 5 minutes.
5. Wash slides in running tap for 1 min.
6. Restain if necessary.
Eric C. Kellar
> ----------
> From: Bruce A Rasmussen[SMTP:brasmuss@osf1.gmu.edu]
> Sent: Wednesday, August 11, 1999 2:39 PM
> To: HistoNet@pathology.swmed.edu
> Subject: Golgi-Cox dark areas.
>
>
> Greetings histo gurus,
>
> Last spring I processed coronal rat brain sections with the Golgi-Cox
> method using the protocol of Kolb/Gibbs. The tissue came out great BUT
> over time the sections are starting to turn black in the medial areas
> threatening our region of interest the hippocampus. We
> store the slides in the dark. Anyone ever see this? Is there anyway to
> stabilize the slides?
>
> Thanks in advance
> Bruce
>
>
>
>
>
> --------------------------------------
> Bruce Rasmussen
> Predoc Fellow in Experimental Neuropsychology
> The Krasnow Institute for Advanced Study
> George Mason University
> Mail Stop 2A1
> Fairfax, VA 22030-4444
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>
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