RE: Golgi-Cox dark areas.

<< Previous Message | Next Message >>
From:"Kellar, Eric" <kellarec@MSX.UPMC.EDU>
To:HistoNet@pathology.swmed.edu, "'Bruce A Rasmussen'" <brasmuss@osf1.gmu.edu>
Reply-To:
Date:Thu, 12 Aug 1999 11:20:11 -0400
Content-Type:text/plain

Pugh et al 1993 described the destaining of sections blackened during and
after the Golgi-Cox method. 
The method is as follows:

1. Bring slides to water.
2. Place slides into Lugol's Iodine for 5 minutes.
3. Wash slides in running tap for 1 min.
4. Place slides into 5% sodium thiosulphate for 5 minutes.
5. Wash slides in running tap for 1 min.
6. Restain if necessary.


Eric C. Kellar

> ----------
> From: 	Bruce A Rasmussen[SMTP:brasmuss@osf1.gmu.edu]
> Sent: 	Wednesday, August 11, 1999 2:39 PM
> To: 	HistoNet@pathology.swmed.edu
> Subject: 	Golgi-Cox dark areas.
> 
> 
> Greetings histo gurus,
> 
> Last spring I processed coronal rat brain sections with the Golgi-Cox
> method using the protocol of Kolb/Gibbs.  The tissue came out great BUT
> over time the sections are starting to turn black in the medial areas
> threatening our region of interest the hippocampus. We
> store the slides in the dark.  Anyone ever see this? Is there anyway to
> stabilize the slides?
> 
> Thanks in advance
> Bruce
> 
> 
> 
> 
> 
> --------------------------------------
> Bruce Rasmussen
> Predoc Fellow in Experimental Neuropsychology
> The Krasnow Institute for Advanced Study
> George Mason University
> Mail Stop 2A1
> Fairfax, VA 22030-4444
> Office:703-993-4358
> Lab:703-993-4369
> Home:703-765-4570
> Fax:703-993-4325
> ---------------------------------------
> 
> 



<< Previous Message | Next Message >>