The runs are being done on a Dako autostainer with our own
reagents.

Bruce W. Brodersen, DVM, PhD   Email:  bbrodersen1@unl.edu
University of Nebraska         Voice:  402 472-1434
Veterinary Diagnostic Center   FAX:    402 472-3094
Lincoln, NE  68583-0907

-----Original Message-----
From: MacDonald, Jennifer [mailto:jmacdonald@sach.org]
Sent: Thursday, August 12, 1999 8:51 AM
To: Histonet; 'Bruce W. Brodersen'
Subject: RE: Failed immunohistochemistry runs




Are you doing them by hand or using automation?  If automated
what
instrument and reagents?

Jennifer

> ----------
> From:         Bruce W. Brodersen[SMTP:bbrodersen1@unl.edu]
> Sent:         Wednesday, August 11, 1999 11:24 AM
> To:   Histonet
> Subject:      Failed immunohistochemistry runs
>
> How often do you have to repeat a run for failed IHC staining
in
> your controls.  It seems we have an inordinant amount of failed
> runs and I haven't pinned it down yet, what the source of the
> problem/s is/are.  Along those same lines, how often do you
make
> new buffers etc.?
>
> TIA
>
> Bruce W. Brodersen, DVM, PhD   Email:  bbrodersen1@unl.edu
> University of Nebraska         Voice:  402 472-1434
> Veterinary Diagnostic Center   FAX:    402 472-3094
> Lincoln, NE  68583-0907
>
>