RE: Failed immunohistochemistry runs
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From: | "MacDonald, Jennifer" <jmacdonald@sach.org> |
To: | "MacDonald, Jennifer" <jmacdonald@sach.org>, Histonet <histonet@pathology.swmed.edu>, "'Bruce W. Brodersen'" <bbrodersen1@unl.edu> |
Reply-To: | |
Date: | Thu, 12 Aug 1999 09:59:11 -0700 |
Content-Type: | text/plain |
Bruce:
We have a Ventana ES and we were having similiar problems with negative
staining of some of our control/patient slides. There was no pattern to the
failed staining. We had a pm done (total change of tubes etc.) and the
problem went away. There was a problem with a pump also that wasn't
apparent until tested during the pm. Just a thought.
Jennifer
> ----------
> From: Bruce W. Brodersen[SMTP:bbrodersen1@unl.edu]
> Sent: Thursday, August 12, 1999 11:28 AM
> To: MacDonald, Jennifer; Histonet
> Subject: RE: Failed immunohistochemistry runs
>
> The runs are being done on a Dako autostainer with our own
> reagents.
>
> Bruce W. Brodersen, DVM, PhD Email: bbrodersen1@unl.edu
> University of Nebraska Voice: 402 472-1434
> Veterinary Diagnostic Center FAX: 402 472-3094
> Lincoln, NE 68583-0907
>
> -----Original Message-----
> From: MacDonald, Jennifer [mailto:jmacdonald@sach.org]
> Sent: Thursday, August 12, 1999 8:51 AM
> To: Histonet; 'Bruce W. Brodersen'
> Subject: RE: Failed immunohistochemistry runs
>
>
>
>
> Are you doing them by hand or using automation? If automated
> what
> instrument and reagents?
>
> Jennifer
>
> > ----------
> > From: Bruce W. Brodersen[SMTP:bbrodersen1@unl.edu]
> > Sent: Wednesday, August 11, 1999 11:24 AM
> > To: Histonet
> > Subject: Failed immunohistochemistry runs
> >
> > How often do you have to repeat a run for failed IHC staining
> in
> > your controls. It seems we have an inordinant amount of failed
> > runs and I haven't pinned it down yet, what the source of the
> > problem/s is/are. Along those same lines, how often do you
> make
> > new buffers etc.?
> >
> > TIA
> >
> > Bruce W. Brodersen, DVM, PhD Email: bbrodersen1@unl.edu
> > University of Nebraska Voice: 402 472-1434
> > Veterinary Diagnostic Center FAX: 402 472-3094
> > Lincoln, NE 68583-0907
> >
> >
>
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