Are you doing them by hand or using automation?  If automated what
instrument and reagents?

Jennifer

> ----------
> From: 	Bruce W. Brodersen[SMTP:bbrodersen1@unl.edu]
> Sent: 	Wednesday, August 11, 1999 11:24 AM
> To: 	Histonet
> Subject: 	Failed immunohistochemistry runs
> 
> How often do you have to repeat a run for failed IHC staining in
> your controls.  It seems we have an inordinant amount of failed
> runs and I haven't pinned it down yet, what the source of the
> problem/s is/are.  Along those same lines, how often do you make
> new buffers etc.?
> 
> TIA
> 
> Bruce W. Brodersen, DVM, PhD   Email:  bbrodersen1@unl.edu
> University of Nebraska         Voice:  402 472-1434
> Veterinary Diagnostic Center   FAX:    402 472-3094
> Lincoln, NE  68583-0907
> 
>