RE: Failed immunohistochemistry runs

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From:Cynthia Favara <cfavara@niaid.nih.gov>
To:"'Bruce W. Brodersen'" <bbrodersen1@unl.edu>, Histonet <histonet@pathology.swmed.edu>
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Date:Thu, 12 Aug 1999 10:30:00 -0400
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I have only had to repeat one run because of control failure. I suspect i
forgot to add the primary. Everything is done by hand, lots of checks to
make sure everything is added correctly. I make buffer as necessary in 10L
quanities. All antibodies are diluted just prior to use. Hope this helps.
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286



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