We optimize other vendors' antibodies for use on the Ventana ES/GenII-we do
no manual staining.  In general we make up our own antibody dilutions but we
do use a few predilutes.  Once they are optimized we put most of them in
dispensers, but we also keep some infrequently ordered antibodies as manual
titrations on the instrument.

Sally Ann Drew, MT(ASCP)
U.of Wis. Hosp. & Clinics
IHC/ISH  Clin./Research Lab
600 Highland Ave. A4/204-2472
Madison, WI 53792-2472
Tel. (608)265-6596
Fax:(608)263-1568

> -----Original Message-----
> From:	Patricia Karlisch [SMTP:PKARLISCH@PSGHS.EDU]
> Sent:	Friday, August 13, 1999 10:53 AM
> To:	Histonet@pathology.swmed.edu
> Subject:	Automatic Staining on IHC
> 
> Dear Histonetters:
>  For those of you who do IHC staining using the Ventana ( a great
> instrument) what do you do for those antibodies that are not offered by
> Ventana for automatic staining.  Do you proceed manually or do you use a
> different autostainer?  Are you using kits or making the antibody
> dilutions
> yourself?  How does this affect your workflow?   The autostainer enhances
> the workflow tremendously but the outliers are labor intensive and I am
> just
> curious how most people handle this .  We do about 80-150 slides a day
> using
> 14-20 different antibodies ( of about 110 different antibodies). About
> 50-70% are done on the Ventana daily depending on what is being ordered.  
> Thank you
> 
> Pat Karlisch
> Geisinger Medical Center
> Danville, Pa  17822
> pkarlisch@psghs.edu
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